Visualization and analysis of unfolded nucleosomes associated with transcribing chromatin
نویسندگان
چکیده
منابع مشابه
Visualization and analysis of unfolded nucleosomes associated with transcribing chromatin.
We have characterized the structure of transcriptionally active nucleosome subunits using electron spectroscopic imaging. Individual nucleosomes were analyzed in terms of total mass, DNA and protein content, while the ensemble of images of active nucleosomes was used to calculate a three-dimensional reconstruction. Transcriptionally active nucleosomes were separated from inactive nucleosomes by...
متن کاملMaking sense of transcribing chromatin
Eukaryotic cells package their genomes into a nucleoprotein form called chromatin. The basic unit of chromatin is the nucleosome, formed by the wrapping of ∼147bp of DNA around an octameric complex of core histones. Advances in genomic technologies have enabled the locations of nucleosomes to be mapped across genomes. This has revealed a striking organisation with respect to transcribed genes i...
متن کاملThe dynamics of individual nucleosomes controls the chromatin condensation pathway: direct AFM visualization of variant chromatin
Université de Lyon; Laboratoire Joliot-Curie, CNRS USR 3010, Ecole Normale Supérieure de Lyon, 46 allée d’Italie, 69364 Lyon Cedex 07, France ; Université de Lyon; Laboratoire de Physique, CNRS UMR 5672, Ecole Normale Supérieure de Lyon, 46 allée d’Italie, 69364 Lyon Cedex 07, France ; Université de Lyon; Laboratoire de Biologie Moléculaire de la Cellule, CNRS UMR 5239, Ecole Normale Supérieure...
متن کاملHistone acetylation is required to maintain the unfolded nucleosome structure associated with transcribing DNA.
Nucleosomes associated with transcribing chromatin of mammalian cells have an unfolded structure in which the normally buried cysteinyl-thiol group of histone H3 is exposed. In this study we analyzed transcriptionally active/competent DNA-enriched chromatin fractions from chicken mature and immature erythrocytes for the presence of thiol-reactive nucleosomes using organomercury-agarose column c...
متن کاملChromatin: Packaging without Nucleosomes
Dinoflagellates are unique among eukaryotes in their unusual 'dinokaryons' - nuclei that lack bulk histones. A new study finds that acquisition of a novel dinoflagellate chromatin protein was an early step in the transition to a nucleus lacking detectable nucleosomes.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.2.321